Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly

Extracellular Alix regulates integrin-mediated cell adhesions and extracellular matrix assembly.

Alix (ALG-2-interacting protein X), a cytoplasmic adaptor protein involved in endosomal sorting and actin cytoskeleton assembly, is required for the maintenance of fibroblast morphology. As Alix has sequence similarity to adhesin in Entamoeba histolytica, and we observed that Alix is secreted, we determined whether extracellular Alix affects fibroblast morphology. Here, we demonstrate that secr...

Physical state of the extracellular matrix regulates the structure and molecular composition of cell-matrix adhesions.

This study establishes that the physical state of the extracellular matrix can regulate integrin-mediated cytoskeletal assembly and tyrosine phosphorylation to generate two distinct types of cell-matrix adhesions. In primary fibroblasts, alpha(5)beta(1) integrin associates mainly with fibronectin fibrils and forms adhesions structurally distinct from focal contacts, independent of actomyosin-me...

Extracellular matrix density promotes EMT by weakening cell-cell adhesions.

Epithelial to mesenchymal transition (EMT), the process during which epithelial cells lose adhesions with neighbouring cells and get converted to migratory and invasive cells, is closely tied to cancer progression. Cancer progression is also marked by increased deposition and cross linking of fibrillar extracellular matrix (ECM) proteins including collagen and fibronectin, which lead to increas...

ICAM-3 regulates lymphocyte morphology and integrin-mediated T cell interaction with endothelial cell and extracellular matrix ligands

Leukocyte activation is a complex process that involves multiple cross-regulated cell adhesion events. In this report, we investigated the role of intercellular adhesion molecule-3 (ICAM-3), the third identified ligand for the beta 2 integrin leukocyte function-associated antigen-1 (LFA-1), in the regulation of leukocyte adhesion to ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), and the 38...

UNC112: A New Regulator of Cell–Extracellular Matrix Adhesions?